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细胞应激生物学国家重点实验室在类器官培养Small发表文章中使用Invigentech(英克)胎牛血清
时间:2020-5-21 21:11:56   来源:   作者:
 

   

  2020-4-27厦门大学生命科学学院细胞应激生物学国家重点实验室发表在Small杂志文章中用微流体制备类器官珠机器与3D打印机配合使用,在类器官培养基中培养类器官珠中使用美国Invigentech(英克),A6903-500胎牛血清进行培养。

发表文章部分内容见下:

1590066460(1).png

Experimental Section 

Kidney Organoid Generation: BALB/c mice (male, 6–8 weeks old) were euthanized by excessive CO2. Then, the kidneys were isolated and cut into small pieces. After washing with cold phosphate buffer saline (PBS) three times, the kidney tissues were digested with 1  mg mL−1 collagenase type I on an orbital shaker at 37 °C for 1–2 h. Each digested tissue suspension was passed through a 100 µm filter. The suspension was centrifuged at 150 × g for 5  min, and the pellet was resuspended in 5  mL of red blood cell lysis buffer for 5  min at room temperature. After centrifugation of the suspension at 150 × g for 5  min, the pellet was resuspended in DMEM/F12 medium. The cells were counted and then mixed with Matrigel (Corning, cat. No. 354 230) at a concentration of 1 × 104 cells µL−1 . Organoid beads were fabricated with a microfluid machine coupled with a 3D printer. After 20  min of solidification in a 37 °C incubator, organoid beads were cultured in organoid medium  containing 20% fetal bovine serum (FBS, Invigentech, cat. No. A6903)。

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Invigentech胎牛血清信息如下:

产品名称货号规格
FBS    Brazil  OriginA6901FBS-500500ml
FBS    Brazil  OriginA6901FBS-100100ml
FBS  Uruguay  OriginA6903FBS-500500ml
FBS  Uruguay  OriginA6903FBS-100100ml
FBS New Zealand OriginA6904FBS-500500ml
FBS New Zealand OriginA6904FBS-100100ml
FBS  Colombia  Origin, Ultra-low EndotoxinA6906FBS-500500ml
FBS  Colombia  Origin, Ultra-low EndotoxinA6906FBS-100100ml
FBS Australia OriginA6907FBS-500500ml
FBS Australia OriginA6907FBS-100100ml


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